The Van Petegem Lab - Publications

THE ION CHANNEL LAB - FILIP VAN PETEGEM

Selected Publications

Yang ZF, Panwar P, McFarlane C, Wietske ET, Campiglio M, Van Petegem F (2022) Structures of the junctophilin/voltage-gated calcium channel interface reveal hot spot for cardiomyopathy mutations. Proc. Natl. Acad. Sci. USA. 119, e2120416119

Woll K, Haji-Ghassemi O, Van Petegem F (2021) Pathological conformations of disease mutant Ryanodine Receptors revealed by cryo-EM. Nature Comm. 12:807.

Ma R, Haji-Ghassemi O, Ma D, Jiang H, Lin L, Yao L, Samurkas A, Li Y, Wang Y, Cao P, Wu S, Zhang Y, Murayama T, Moussian B, Van Petegem F*, Yuchi Z*. (2020) Structural basis for diamide modulation of ryanodine receptor. Nat Chem Biol. 16,1246-1254. *co-corresponding authors.

Haji-Ghassemi O, Yuchi Z, Van Petegem F (2019) The Cardiac Ryanodine Receptor Phosphorylation Hot Spot Embraces PKA in a Phosphorylation-Dependent Manner. Molecular Cell. 75, 1-14.

Gardill B, Tung C-C, Van Petegem F (2019) Crystal structures of Ca2+-calmodulin bound to NaV C-terminal regions suggest role for EF-hand domain in binding and inactivation. Proc. Natl. Acad. Sci. USA. 116,10763-10722.

Wang K, Holt C, Lu J, Brohus M, Larsen KT, Overgaard MT, Wimmer R, Van Petegem F (2018)Arrhythmia mutations in calmodulin cause conformational changes that affect interactions with the cardiac voltage-gated calcium channel. Proc. Natl. Acad. Sci. USA 115:E10556-E10565.

Wong King Yuen S, Campiglio M, Tung CC, Flucher BE, Van Petegem F (2017) Structural insights into binding of STAC proteins to voltage-gated calcium channels. Proc. Natl. Acad. Sci. USA.114:E9520-E9528.

Das S, Gilchrist J, Bosmans F, Van Petegem F (2016) Binary architecture of the NaV1.2-beta2 signaling complex. Elife e10960.

Yuchi Z, Yuen SM, Lau K, Underhill AQ, Cornea RL, Fessenden JL, Van Petegem F (2015) Crystal structures of ryanodine receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant. Nature Comm. 6:7947

Lau K, Van Petegem F (2014) Crystal structures of wild type and disease mutant forms of the Ryanodine Receptor SPRY2 domain. Nature Comm. 5:5397.

Kimlicka L, Lau K, Tung CC, Van Petegem F (2013) Disease Mutations in the Ryanodine Receptor N-terminal region couple to a mobile intersubunit interface. Nature Comm. 4:1506.

Sarhan MF, Tung CC, Van Petegem F*, Ahern CA* (2012) Crystallographic basis for calcium regulation of sodium channels. Proc Nat Acad Sci USA 109,3558-3363. * shared corresponding authorship

Tung CC, Lobo PA, Kimlicka L, Van Petegem F (2010) The N-terminal disease hot spot of Ryanodine Receptors forms a cytoplasmic vestibule. Nature. 468, 585-588.

ALL PUBLICATIONS BY FILIP VAN PETEGEM

2022

O’Connor TN, van den Bersselaar LR, Chen YS, Nicolau S, Simon B, Huseth A, Todd JJ, Van Petegem F, Sarkozy A, Goldberg MF, Voermans NC, Dirksen RT (2022) RyR-1-Related Diseases International Workshop: From Mechanisms to Treatments. J. Neuromusc. Dis. In press.

Pesch YY, Dang V, Fairchild MJ, Islam F, Camp D, Kaur P, Smendziuk CM, Messenberg A, Carr R, McFarlane CR, Musso PY, Van Petegem F, Tanentzapf G (2022). Gap junctions mediate discrete regulatory steps during fly spermatogenesis. PloS Genet. 18(9):e1010417

Rayani K, Hantz ER, Haji-Ghassemi O, Li AY, Spuches AM, Van Petegem F, Solaro RJ, Lindert S, Tibbits GF (2022) The effect of Mg2+ and Ca2+ binding to cardiac troponin C in hypertrophic cardiomyopathy associated TNNC1 variants. FEBS J. In press.

Willegems K, Eldstrom J, Kyriakis E, Ataei F, Sahakyan H, Dou Y, Russo S, Van Petegem F*, Fedida F* (2022) Structural and electrophysiological basis for the modulation of KCNQ1 channel currents by ML277. Nat Commun 13:3760 *co-corresponding authors

Qin J, Zhang J, Lin L, Haji-Ghassemi O, Lin Z, Woycechowsky KJ, Van Petegem F, Zhang Y, Yuchi Z (2022) Structures of PKA-phospholamban complexes reveal a mechanism of familial dilated cardiomyopathy. Elife. 11:e75346

Yang ZF, Panwar P, McFarlane C, Wietske ET, Campiglio M, Van Petegem F (2022) Structures of the junctophilin/voltage-gated calcium channel interface reveal hot spot for cardiomyopathy mutations. Proc. Natl. Acad. Sci. USA. 119, e2120416119

Ng L, Li YX, Van Petegem F, Accili EA (2022) Altered cyclic nucleotide-binding and pore opening in a diseased human HCN4 channel. Biophys J. 12:1166-1183

Arslanova A, Shaffaattalab S, ye K, Asghari P, Lin L, Kim B, Roston TM, Hove-Madsen L, Van Petegem F, Sanatani S, Moore E, Lynn F, Sondergaard M, Luo Y, Chen SRW, Tibbits GF (2022) Using hiPSC-CMs to Examine Mechanisms of Catecholaminergic Polymorphic Ventricular Tachycardia. Curr Protoc. 1:e320

Rossi D, Lorenzini S, Pierantozzi E, Van Petegem F, Amadsun DO, Sorrentino V (2022) Multiple regions of junctin drive interaction with calsequestrin-1 and localization at triads in skeletal muscle. J. Cell Sci. 135:jcs259185

Zhang J, Singh DP, Ko CY, Nikolaienko R, Wong King Yuen SM, Schwarz JA, Treinen LM, Tung CC, Rozman K, Svensson B, Aldrich CC, Zima AV, Thomas DD, Bers DM, Launikonis BS, Van Petegem F, Cornea RL (2022) Cardiac ryanodine receptor N-terminal region biosensors identify novel inhibitors via FRET-based high-throughput screening. J.Biol.Chem. 15:101412

2021

Grondin JM, Dejean G, Van Petegem F, Brumer H (2021) Cell-surface xyloglucan recognition and hydrolysis by the human gut commensal Bacteroides uniformis Apppl Environ Microbiol. In Press

Scortecci JF, Molday LL, Curtis SB, Garces FA, Panwar P, Van Petegem F, Molday RS (2021) Cryo-EM structures of the ABCA4 importer reveal mechanisms underlying substrate binding and Stargardt disease. Nature Commun. 12:5902

Woll K, Van Petegem F (2021) Calcium release channels: structure and function of IP3 Receptors and Ryanodine Receptors. Physiol.Rev. doi: 10.1152/physrev.00033.2020

Rufenach B, Van Petegem F (2021) Structure and function of STAC proteins: Calcium channel modulators and critical components of muscle excitation-contraction coupling. J.Biol.Chem. 297:100874

van den Bersselaar LR, Greven T, Bulger T, Voermans NC, Van Petegem F, Schiemann AH, Parker R, Burling SM, Jungbluth H, Stowell KM, Kamsteeg EG, Snoeck MMJ (2021) RYR1 variant c.38T>G, p.Leu13Arg causes hypersensitivity of the ryanodine receptor-1 and is pathogenic for malignant hyperthermia. Br.J.Anaesth. 127:e63-e65

Jain N, Tamura K, Dejean G, Van Petegem F, Brumer H (2021) Orthogonal Active-Site Labels for Mixed-Linkage endo-ß-Glucanases. ACS Chem Biol. In Press.

Scala M, Stephanie E, Sultan T, De Waele J, Panciroli M, Maroofian R, Striano P, Van Petegem F, Houlden H, Bosmans F (2021) Homozygous SCN1B variants causing early infantile epileptic encephalopathy 52 affect voltage-gated sodium channel function. Epilepsia. 62, e82-e87.

Safabaksh S, Panwar P, Barichello S, Sangha SS, Hanson PJ, Van Petegem F, Laksman Z (2021) The role of phosphorylation in atrial fibrillation: a focus on mass spectrometry approaches. Cardiovasc. Res. Doi: 10.1093/cvr/cvab095

Tamura K, Dejean G, Van Petegem F, Brumer H (2021) Distinct protein architectures mediate species-specific beta-glucan binding and metabolism in the human gut microbiota. J. Biol. Chem. 296:100415

Rayani K, Seffernick J, Li AY, David JP, Spuches AM, Van Petegem F, Solaro RJ, Lindert S, Tibbits GF (2021) Binding of calcium and magnesium to human cardiac Troponin C. J. Biol. Chem 296:100350

Woll K, Haji-Ghassemi O, Van Petegem F (2021) Pathological conformations of disease mutant Ryanodine Receptors revealed by cryo-EM. Nature Comm. 12:807

2020

Genovese I, Giamogante F, Barazzuol L, Battista T, Fiorillo A, Vicario M, D’Alessandro G, Cipriani R, Limatola C, Rossi D, Sorrentino V, Poser E, Mosca L, Squitieri F, Perluigi M, Arena A, Van Petegem F, Tito C, Fazi F, Giorgi C, Cali T, Ilari A, Colotti G (2020) Sorcin is an early marker of neurodegeneration, Ca2+ dysregulation and Endoplasmic Reticulum Stress associated to neurodegenerative diseases. Cell Death and Disease. 11:861

Llongueras JP, Das S, De Waele J, Capulzini L, Sorgente A, Van Petegem F*, Bosmans F* (2020) Biophysical Investigation of Sodium Channel Interaction with ß-Subunit Variants Associated with Arrhythmias. Bioelectricity 2,269-278 *co-corresponding authors

Ibarra Moreno CA, Kraeva N, Zvaritch E, Figueroa L, Rios E, Biesecker L, Van Petegem F, Hopkins PM, Riazi S (2020) A multi-dimensional analysis of genotype-phenotype discordance in malignant hyperthermia susceptibility. Br J Anaesth. 2020 Aug 26:S0007-0912(20)30633-4. doi: 10.1016/j.bja.2020.07.042.

Chamberlain TC, Cheung ST, Yoon JSJ, Ming-Lum A, Gardill BR, Shakibakho S, Dzananovic E, Ban F, Samiea A, Jawanda K, Priatel J, Krystal G, Ong CJ, Cherkasov A, Andersen RJ, McKenna SA, Van Petegem F, Mui AL (2020) Interleukin-10 and Small Molecule SHIP1 Allosteric Regulators Trigger Anti-inflammatory Effects through SHIP1/STAT3 Complexes. iScience. 23(8):101433

Ma R, Haji-Ghassemi O, Ma D, Jiang H, Lin L, Yao L, Samurkas A, Li Y, Wang Y, Cao P, Wu S, Zhang Y, Murayama T, Moussian B, Van Petegem F*, Yuchi Z*. (2020) Structural basis for diamide modulation of ryanodine receptor. Nat Chem Biol. 16,1246-1254. *co-corresponding authors.

Rufenach B, Christy D, Flucher BE, Bui JM, Gsponer J, Campiglio M, Van Petegem F (2020) Multiple Sequence Variants in STAC3 Affect Interactions with CaV 1.1 and Excitation-Contraction Coupling. Structure. 28(8):922-932

Holt C, Hamborg L, Lau K, Brohus M, Sørensen AB, Larsen KT, Sommer C, Van Petegem F*, Overgaard MT*, Wimmer R*. (2020) The arrhythmogenic N53I variant subtly changes the structure and dynamics in the calmodulin N-terminal domain, altering its interaction with the cardiac ryanodine receptor. J. Biol. Chem. 295(22):7620-7634 * co-corresponding authors

Dejean G, Tamura K, Cabrera A, Jain N, Pudio N, Pereira G, Viborg A, Van Petegem F, Martens E, Brumer H (2020) Synergy between cell-surface glycosidases and glycan-binding proteins dictates the utilization of specific beta(1,3)-glucans by human gut Bacteroides. mBio. 11:e00095-20

Stringer RN, Jurkovicova-Tarabova B, Huang S, Haji-Ghassemi O, Idoux R, Liashenko A, Souza IA, Rzhepetskyy Y, Lacinova L, Van Petegem F, Zamponi GW, Pamphlett R, Weiss N (2020) A rare CACNA1H variant associated with amyotrophic lateral sclerosis causes complete loss of Cav3.2 T-type channel activity. Mol. Brain. 13:33

Wang K, Brohus M, Holt C, Overgaard MT, Wimmer R, Van Petegem F (2020) Arrhythmia mutations in calmodulin can disrupt cooperativity of Ca2+ binding and cause misfolding. J. Physiol. 598(6):1169-1186

Tung M, Van Petegem F, Lauson S, Collier A, Hodkinson K, Fernandez B, Connors S, Leather R, Sanatani S, Arbour L (2020) Cardiac arrest in a mother and daughter and the identification of a novel RYR2 variant predisposing to low penetrant Catecholaminergic Polymorphic Ventricular Tachycardia in a 4 generation Canadian family. Mol. Genet. Genomic Med. 8(4) e1151

Gardill B, Huang J, Tu L, Van Petegem F, Oxenoid K, Thomson C (2020) Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins. Sci. Rep. 10(1):1130

Asghari P, Scriven DR, Ng M, Panwar P, Chou KC, Van Petegem F, Moore ED (2020) Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modification. Elife. 9. pii: e51602.

Round P, Das S, Wu TS, Wahala K, Van Petegem F, Hammond GL (2020) Molecular interactions between sex hormone-binding globulin and non-steroidal ligands that enhance androgen action. J. Biol. Chem. 295, 1202-1211

2019

Ng L, Zhuang M, Van Petegem F, Li YX, Accili EA (2019) Binding and structural asymmetry governs ligand sensitivity in a cyclic nucleotide-gated ion channel. J.Gen.Physiol. 151,1190-1212

Mellor GJ, Panwar P, Lee AK, Steinberg C, Hathaway J, Bartels C, Christian S, Balaji S, Roberts JD, Simpson CS, Boczek NJ, Tester DJ, Radbill AE, Mok N-S, Hamilton R, Kaufman E, Eugenio PL, Weiss R, January C, McDaniel GM, Leather RA, Erickson C, Falik S, Behr ER, Wilde AAM, Sanatani S, Ackerman MJ, Van Petegem F, Krahn AD, Laksman Z (2019) Type 8 Long QT Syndrome: Pathogenic Variants in CACNA1C-Encoded Cav1.2 Cluster in STAC Protein Binding Site. Europace. 21,1725-1732

Haji-Ghassemi O, Yuchi Z, Van Petegem F (2019) The Cardiac Ryanodine Receptor Phosphorylation Hot Spot Embraces PKA in a Phosphorylation-Dependent Manner. Molecular Cell. 75, 1-14

Gardill B, Tung C-C, Van Petegem F (2019) Crystal structures of Ca2+-calmodulin bound to NaV C-terminal regions suggest role for EF-hand domain in binding and inactivation. Proc. Natl. Acad. Sci. USA. 116, 10761-10772

Tamura K, Foley MH, Gardill BR, Dejean G, Schnizlein M, Bahr CME, Louise Creagh A, Van Petegem F, Koropatkin NM, Brumer H (2019) Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Cell Mol. Life Sci. 76,4319-4340

Alvarado FJ, Bos JM, Yuchi Z, Valdivia CR, Hernandez JJ, Zhao YT, Henderlong DS, Chen Y, Booher TR, Marcou C, Van Petegem F, Ackerman MJ, Valdivia HH (2019) Cardiac Hypertrophy and Arrhythmia in Mice Harboring a Novel RyR2 Missense Variant Associated with Hypertrophic Cardiomyopathy. J. Clin. Invest. Insight 5:e1265441

Shafaattalab S, Li AY, Lin E, Stevens CM, Dewar L, Lynn F, Sanatani S, Laksman Z, Van Petegem F, Madsen LH, Tieleman P, Davis J, Tibbits GF (2019) Arrhythmogenic cardiac thin filament mutations as a potential cause of sudden cardiac death in infants: post-mortem in vitro analyses to determine causality. Proc. Natl. Acad. Sci. USA.116, 6969-6974

Haji-Ghassemi O, Muller-Loennnies S, Brooks CL, MacKenzie CR, Caveney N, Van Petegem F, Brade L, Kosma P, Brade H, Evans SV (2019) Subtle changes in the combining site of the Chlamydiaceae-specific mAb S25-23 increase antibody-carbohydrate binding affinity by an order of magnitude. Biochemistry. 58, 714-726

Brohus M, Sondergaard MT, Chen SR, Van Petegem F, Overgaard MT (2019) Ca2+ dependent calmodulin binding to cardiac ryanodine receptor (RyR2) calmodulin-binding domains. Biochemical J. 476, 193-209

2018

Wang K, Holt C, Lu J, Brohus M, Larsen KT, Overgaard MT, Wimmer R, Van Petegem F (2018) Arrhythmia mutations in Calmodulin cause conformational changes that affect interactions with he cardiac voltage-gated calcium channel. Proc. Natl. Acad. Sci USA. 115(45):E10556-E10565

Pancaroglu R, Van Petegem F (2018) Calcium channelopathies: structural and functional insights into disorders of the muscle excitation-contraction complex. Ann. Rev. Genet. 52:373-396

Gardill B, Rivera-Acevedo RE, Tung C-C, Okon M, McIntosh LP, Van Petegem F (2018). The voltage-gated sodium channel EF-hands forms an interaction with the III-IV linker that is disturbed by disease-causing mutations. Sci. Rep.8(1):4483

Campiglio M, Coste de Bagneaux P, Ortner NJ, Tuluc P, Van Petegem F, Flucher BE (2018) STAC proteins associate to the IQ domain of CaV1.2 and inhibit calcium-dependent inactivation. Proc. Natl. Acad. Sci. USA 115, 1376-1381.

Roston T*, Haji-Ghassemi O*, LaPage MJ, Batra A, Bar-Cohen Y, Anderson C, Lau YR, Maginot K, Gebauer RA, Etheridge SP, Potts JE, Van Petegem F, Sanatani S (2018) Catecholaminergic polymorphic ventricular tachycardia patients with multiple genetic variants in the PACES CPVT Registry. Plos One. 13(11):e0205925

2017

Wong King Yuen SM, Campiglio M, Tung CC, Flucher BE, Van Petegem F (2017) Structural insights into binding of STAC proteins to voltage-gated calcium channels. Proc. Natl. Acad. Sci. USA. In Press.

Liu Y, Wei J, Wong King Yuen SM, Sun B, Tang Y, Wang R, Van Petegem F, Chen SRW (2017) CPVT-associated cardiac ryanodine receptor mutation G357S with reduced penetrance impairs Ca2+ release termination and diminishes protein expression. PLoS One 12,e0184177

Roston T, Yuchi Z, Kannankeril PJ, Hathaway J, Vinocur JM, Etheridge SP, Potts JE, Maginot KR, Salerno JC, Cohen M, Hamilton RM, Pflaumer A, Mohammed S, Kimlicka L, Kanter RJ, LaPage MJ, Collins KK, Gebauer RA, Temple JD, Batra AS, Erickson C, Miszczak-Knecht M, Kubus P, Bar-Cohen Y, Kantoch M, Thomas VC, Hessling G, Anderson C, Young ML, Choi SHJ, Cabrera Ortega M, Lau YR, Johnsrude CL, Fournier A, Van Petegem F, Sanatani S (2017). The Clinical and Genetic Spectrum of Catecholaminergic Polymorphic Ventricular Tachycardia: Identifying Predictors of Arrhythmia in an International Multicenter Registry. Europace. In Press.

Sondergaard MT, Liu Y, Larsen KT, Nani A, Tian X, Holt C, Wang R, Wimmer R, Van Petegem F, Fill M, Chen SR, Overgaard MT (2017) The Arrhythmogenic Calmodulin p.Phe142Leu Mutation Impairs C-domain Ca2+-binding but not Calmodulin-dependent Inhibition of the Cardiac Ryanodine Receptor. J. Biol. Chem. 292,1385-1395.

McGregor N, Yin V, Ching-Chieh T, Van Petegem F, Brumer H (2017) Crystallographic insight into the evolutionary origins of xyloglucan endo-transglycosylases and endo-hydrolases. The Plant J 89(4):651-670.

Roston T, Van Petegem F, Sanatani S (2017) Catecholaminergic Polymorphic Ventricular Tachycardia: a model for Genotype-Specific Therapy. Curr.Opinion in Cardiol. 32,78-85

2016

Das S, Gilchrist J, Bosmans F, Van Petegem F (2016) Binary architecture of the Nav1.2-β2 signaling complex. eLife 5: e10960

Alzayady KJ, Wang L, Chadrasekhar R, Wagner II LE, Van Petegem F, Yule D (2016) Defining the Stoichiometry of Inositol 1,4,5-Trisphosphate Binding Required to Initiate Ca2+ Release. Science Signaling. 9(422):ra35.

Vashchenko G, Das S, Moon KM, Rogalski JC, Taves MD, Soma KK, Van Petegem F, Foster LJ, Hammond GL (2016) Identification of avian corticosteroid-binding globulin (SerpinA6) reveals the molecular basis of evolutionary adaptations in SerpinA6 structure and function as a steroid-binding protein. J. Biol. Chem. 291,11300-11312.

Yuchi Z, Van Petegem F (2016) Ryanodine Receptors under the Magnifying Lens: insights and limitations of cryo-electron microscopy and X-ray crystallography studies. Cell Calcium. 59, 209-227

Stevens C., Rayani K, Genge C, Singh G, Liang B, Roller J, Li C, Li A, Tieleman P, Van Petegem F, Tibbits G (2016) Functional Characterization of Cardiac and Slow Skeletal Troponin C Paralogs in Zebrafish by MD Simulation and Isothermal Titration Calorimetry. Biophysical J. 111, 38-49.

Ng L, Putrenko I, Baronas V, Van Petegem F, Accili E (2016) Cyclic purine and pyrimidine nucleotides bind to the HCN2 ion channel and variably promote C-terminal domain interactions and opening. Structure. 24,1629-1642

Van Petegem F (2016) “How to open a Ryanodine Receptor”. Cell Research. 26,1073-1074.

Roston T, Guo W, Krahn AD, Wang R, Van Petegem F, Sanatani S, Chen SR, Lehman A (2016) A Novel RYR2 Loss-of-Function Mutation (I4855M) is Associated with Left Ventricular Non-compaction and Atypical Catecholaminergic Polymorphic Ventricular Tachycardia. J. Electrocardiol. In press.

2015

Xiao Z, Guo W, Wong King Yuen S, Wang R, Zhang L, Van Petegem F, Chen SR (2015) The H29D mutation does not enhance cytosolic Ca2+ activation of the cardiac Ryanodine Receptor. Plos One 10:e0139058.

Brath U, Swamy SI, Veiga AX, Tung CC, Van Petegem F, Erdelyi M (2015) Paramagnetic Ligand Tagging to Identify Protein Binding Sites. J. Am. Chem. Soc.137,11391-11398.

Yuchi Z, Wong King Yuen S, Lau K, Underhill AQ, Cornea R, Fessenden J, Van Petegem F (2015) Crystal structures of Ryanodine Receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant. Nature Comm. 6,7947.

Liu Y, Xiao Z, Wang R, Guo W, Zhang JZ, Mi T, Wang Y, Jones PP, Van Petegem F, Chen SRW (2015) Roles of the NH2-terminal Domains of Cardiac Ryanodine Receptor in Ca2+ Release Activation and Termination. J. Biol. Chem. 290, 7736-7743.

Van Petegem F (2015). Ryanodine Receptors: Allosteric Ion Channel Giants. J. Mol. Biol. 427,31-53

2014

Lau K, Van Petegem F (2014) Crystal structures of wild type and disease mutant forms of the Ryanodine Receptor SPRY2 domain.Nature Comm. 5:5397

Trane AE, Pavlov D, Sharma A, Saqib U, Lau K, Van Petegem F, Minshall RD, Roman LJ, Bernatchez PN (2014) Deciphering the binding of caveolin-1 to clien protein endothelial nitric-oxide synthase (eNOS): scaffolding subdomain identification, interaction modeling, and biological significance. J Biol Chem 289, 13273-13283.

Lau K, Chan MM, Van Petegem F* (2014) Lobe-Specific Calmodulin Binding to Different Ryanodine Receptor Isoforms. Biochemistry 53, 932-946

Brath U, Lau K, Van Petegem F*, Erdelyi M (2014) Mapping the sevoﬂurane-binding sites of calmodulin. Pharma Res Per, 2, e00025 doi:10.1002/prp2.25.

2013

Gilchrist J, Das S, Van Petegem F*, Bosmans F* (2013) Crystallographic insights into sodium-channel modulation by the β4 subunit. Proc. Natl. Acad. Sci. USA. 110, E5016-5024. *shared corresponding authorship

Zhong X, Liu Y, Zhu L, Meng X, Wang R, Van Petegem F, Wagenknecht T, Chen SR, Liu Z (2013) Conformational dynamics inside amino-terminal disease hotspot of ryanodine receptor. Structure 21, 2051-2060.

Ellis SJ, Goult BT, Fairchild MJ, Harris NJ, Long J, Lobo P, Czerniecki S, Van Petegem F, Schöck F, Peifer M, Tanentzapf G. (2013) Talin autoinhibition is required for morphogenesis. Curr Biol 23,1825-1833.

Amador FJ, Kimlicka L, Stathopulos PB, Gasmi-Seabrook GM, Maclennan DH, Van Petegem F*, Ikura M* (2013) Type 2 ryanodine receptor domain A contains a unique and dynamic α-helix that transitions to a β-strand in a mutant linked with a heritable cardiomyopathy. J Mol Biol 425,4034-4046 *Shared corresponding authorship.

Kimlicka L, Tung CC, Carlsson AC, Lobo PA, Yuchi Z, Van Petegem F (2013) The cardiac ryanodine receptor N-terminal region contains an anion binding site that is targeted by disease mutations. Structure 21,1440-1449.

Liu Y, Kimlicka L, Hiess F, Tian X, Wang R, Zhang L, Jones PP, Van Petegem F, Chen SR (2013) The CPVT-associated RyR2 mutation G230C enhances store overload-induced Ca2+ release and destabilizes the N-terminal domains. Biochem J 454,123-131

Kimlicka L, Lau K, Tung CC, Van Petegem F (2013) Disease Mutations in the Ryanodine Receptor N-terminal region couple to a mobile intersubunit interface. Nature Comm. 4:1506.

2012

Van Petegem F, Lobo PA, Ahern CA (2012) Seeing the forest through the trees: towards a unified view on physiological calcium regulation of voltage-gated sodium channels. Biophys J 103, 2243-2251.

Van Petegem F (2012) Ryanodine Receptors: Structure and Function. J. Biol. Chem.287, 31624-31632

Yuchi Z, Lau K, Van Petegem F (2012) Disease Mutations in the Ryanodine Receptor Central Region: Crystal Structures of a Phosphorylation Hot Spot Domain. Structure. 20, 1201-1211.

Liu X, Chen M, Lobo P, An J, Grace Cheng SW, Moradian A, Morin GB, Van Petegem F*, Jiang X* (2012) Molecular and structural characterization of the SH3 domain of AHI-1 in regulation of cellular resistance of BCR-ABL(+) chronic myeloid leukemia cells to tyrosine kinase inhibitors. Proteomics 12 ,2094-2106 *co-corresponding authors

Sarhan MF, Tung CC, Van Petegem F*, Ahern CA* (2012) Crystallographic basis for calcium regulation of sodium channels. Proc Nat Acad Sci USA 109,3558-3363. *co-corresponding authors

Chow SS, Van Petegem F, Accili EA (2012) Energetics of cAMP binding to HCN terminus reveal negative cooperativity. J. Biol. chem. 287, 600-606.

2011

Kimlicka L, Van Petegem F(2011) The structural biology of ryanodine receptors. Science China Life Sciences. 54, 712-724. (review article)

Lobo PA, Kimlicka L, Tung CC, Van Petegem F (2011) The RyR2 exon3 deletion is rescued by beta strand switching. Structure 19,90-98.

Yuchi Z, Van Petegem F (2011) Common allosteric mechanisms between ryanodine and inositol-1,4,5-trisphosphate receptors. Channels 5, 120-123.

2010

Tung CC, Lobo PA, Kimlicka L, Van Petegem F (2010) The N-terminal disease hot spot of Ryanodine Receptors forms a cytoplasmic vestibule. Nature. 468, 585-588.

Kim EY, Rumpf CH, Van Petegem F, Arant RJ, Findeisen F, Cooley ES, Isacoff EY, Minor DL Jr. (2010) Multiple C-terminal tail Ca(2+)/CaMs regulate Ca(V)1.2 function but do not mediate channel dimerization. EMBO J. 29,3924-3938.

Okamoto S, Van Petegem F, Patrauchan MA, Eltis LD (2010) AnhE, a metallochaperone involved in the maturation of a cobalt-dependent nitrile hydratase. J Biol Chem. 285, 25126-25133.

Janssens ME, Geysen D, Broos K, De Goeyse I, Robbens J, Van Petegem F, Timmermans J-P, Guisez Y (2010) Folding properties of the hepatitis B core as a carrier protein for vaccination research. Amino Acids. 38, 1617-1626.

2009

Cheung TYS, Fairchild M, Zarivach R, Tanentzapf G, Van Petegem F (2009) Crystal Structure of the Talin Integrin Binding Domain 2. J. Mol. Biol. 387, 787-793.

Sarhan MS, Van Petegem F, Ahern CA (2009). A double tyrosine motif in the cardiac sodium channel Domain III-IV linker couples calcium dependent calmodulin binding to inactivation gating. J Biol Chem, 284, 33265-33274

Lobo PA, Van Petegem F (2009). Crystal structures of the N-terminal domains of cardiac and skeletal muscle ryanodine receptors: insights into disease mutations. Structure, 17,1505-1514.

2008

De Vos D, Xu Y, Aerts T, Van Petegem F, Van Beeumen JJ (2008). Crystal structure of Sulfolobus acidocaldarius aspartate carbamoyltransferase in complex with its allosteric activator CTP. Biochem Biophys Res Commun., 372, 40-44

Kim EY, Rumpf CH, Fujiwara Y, Cooley ES, Van Petegem F, Minor DL (2008). Structures of Ca_V2 Ca²⁺/CaM-IQ domain complexes reveal binding modes that underlie calcium-dependent inactivation and facilitation. Structure, 16, 1455-1467.

Van Petegem F, Duderstadt KE, Clark KA, Wang M, Minor DL (2008). Alanine-scanning mutagenesis defines a conserved energetic hotspot in the Ca_Vα AID-Ca_Vβ interaction site that is critical for channel modulation. Structure, 16, 280-294.

2005-2007

Van Petegem F, De Vos D, Savvides S, Vergauwen B, Van Beeumen J (2007). Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: Crystallographic analysis of a glutathione amide J. Mol. Biol., 374, 883-889

Van Petegem F, Minor DL (2006). The structural biology of voltage-gated calcium channel function and regulation. Biochem. Soc. Trans, 34, 887-893.

Van Petegem F, Chatelain FC, Minor DL (2005) Insights into voltage-gated calcium channel regulation from the structure of the Ca_V1.2 IQ domain-Ca²⁺/calmodulin complex. Nature Struct. Mol. Biol., 12, 1108-1115

2003-2004

Van Petegem F, Clark KA, Chatelain FC, Minor DL Jr. (2004) Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain. Nature 429, 671-675.

Dumortier C, Fitch J, Van Petegem F, Vermeulen W, Meyer TE, Van Beeumen J, Cusanovich MA. (2004) Protein dynamics in the region of the sixth ligand methionine revealed by studies of imidazole binding to Rhodobacter capsulatus cytochrome c2 hinge mutants. Biochemistry, 43, 7717-7724.

De Vos D, Van Petegem F, Remaut H, Legrain C, Glansdorff N, Van Beeumen J. (2004) Crystal structure of T state aspartate carbamoyltransferase of the hyperthermophilic archaeon Sulfolobus acidocaldarius. J. Mol. Biol. 339, 887-900.

Aghajari N, Van Petegem F, Villeret V, Chessa JP, Gerday C, Haser R, and Van Beeumen J. (2003). Crystal structures of a psychrophilic metalloprotease provide new insights into catalysis by cold-adapted proteases. Proteins: Struct. Funct. Genet. 50, 636-647.

2000-2002

Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, and Van Beeumen J (2002). The structure of a cold-adapted family 8 xylanase at 1.3 Å resolution, structural adaptations to cold and investigation of the active site. J. Biol. Chem., 278, 7531-7539

Van Petegem F, Vandenberghe I, Bhat MK, and Van Beeumen J (2002). Atomic resolution structure of the major endoglucanase from normal Thermoascus aurantiacus. Biochem. Biophys. Res. Commun. 296, 161-166.

Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, and Van Beeumen J (2002). Crystallization and preliminary X-ray analysis of a xylanase from the psychrophile Pseudoalteromonas haloplanktis. Acta Cryst. D., 58, 1494-1496.

Vergauwen B, Van Petegem F, Remaut H, Pauwels F, and Van Beeumen J (2002). Crystallization and preliminary X-ray crystallographic analysis of glutathione amide reductase from Chromatium gracile. Acta Cryst. D., 58, 339-340.

Van Petegem F, Contreras H, Contreras R, and Van Beeumen J (2001). Trichoderma reesei alpha-1,2-mannosidase: structural basis for the cleavage of four consecutive mannose residues. J. Mol. Biol., 312, 157-165.

Georlette D, Jonsson ZO, Van Petegem F, Chessa J, Van Beeumen J, Hubscher U, Gerday C (2000). A DNA ligase from the psychrophile Pseudoalteromonas haloplanktis gives insights into the adaptation of proteins to low temperatures. Eur. J. Biochem. 267, 3502-3512.

keywords: cardiac arrhythmia, epilepsy, ion channel, CPVT, Dravet syndrome, malignant hyperthermia, central core disease, excitation-contraction coupling, ryanodine receptor, sodium channel, calcium channel, voltage-gated sodium channel, calmodulin, X-ray crystallography, ryanodine, RyR, RyR1, RyR2, RyR3, high resolution, structure, arrhythmia, genetic disorder, ITC, isothermal titration calorimetry, Brugada syndrome, Long QT syndrome, arrhythmia, channel, electrophysiology, GEFS+, Vancouver, UBC, Biochemistry, Canada
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Van Petegem Lab © 2012, updated: May 2019