Selected Publications

Das S, Gilchrist J, Bosmans F, Van Petegem F (2016) Binary architecture of the NaV1.2-beta2 signaling complex. Elife e10960.

 

Yuchi Z, Yuen SM, Lau K, Underhill AQ, Cornea RL, Fessenden JL, Van Petegem F (2015) Crystal structures of ryanodine receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant. Nature Comm. 6:7947

 

Lau K, Van Petegem F (2014) Crystal structures of wild type and disease mutant forms of the Ryanodine Receptor SPRY2 domain. Nature Comm. 5:5397.

 

Gilchrist J, Das S, Van Petegem F*, Bosmans F* (2013) Crystallographic insights into sodium-channel modulation by the β4 subunit. Proc. Natl. Acad. Sci. USA. 110, E5016-5024. *shared corresponding authorship

 

Kimlicka L, Lau K, Tung CC, Van Petegem F (2013) Disease Mutations in the Ryanodine Receptor N-terminal region couple to a mobile intersubunit interface. Nature Comm. 4:1506.

 

Sarhan MF, Tung CC, Van Petegem F*, Ahern CA* (2012) Crystallographic basis for calcium regulation of sodium channels. Proc Nat Acad Sci USA 109,3558-3363. * shared corresponding authorship

 

Tung CC, Lobo PA, Kimlicka L, Van Petegem F (2010) The N-terminal disease hot spot of Ryanodine Receptors forms a cytoplasmic vestibule. Nature. 468, 585-588.

 

Van Petegem F, Chatelain FC, Minor DL (2005) Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex. Nature Struct. Mol. Biol., 12, 1108-1115

 

Van Petegem F, Clark KA, Chatelain FC, Minor DL Jr. (2004) Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain. Nature 429, 671-675.

 

ALL PUBLICATIONS BY F. VAN PETEGEM

2016

Das S, Gilchrist J, Bosmans F, Van Petegem F (2016) Binary architecture of the Nav1.2-β2 signaling complex. eLife 5: e10960

 

Alzayady KJ, Wang L, Chadrasekhar R, Wagner II LE, Van Petegem F, Yule D (2016) Defining the Stoichiometry of Inositol 1,4,5-Trisphosphate Binding Required to Initiate Ca2+ Release. Science Signaling. 9(422):ra35.

 

Vashchenko G, Das S, Moon KM, Rogalski JC, Taves MD, Soma KK, Van Petegem F, Foster LJ, Hammond GL (2016) Identification of avian corticosteroid-binding globulin (SerpinA6) reveals the molecular basis of evolutionary adaptations in SerpinA6 structure and function as a steroid-binding protein. J. Biol. Chem. 291,11300-11312.

 

Yuchi Z, Van Petegem F (2016) Ryanodine Receptors under the Magnifying Lens: insights and limitations of cryo-electron microscopy and X-ray crystallography studies.  Cell Calcium. 59, 209-227

 

Stevens C., Rayani K, Genge C, Singh G, Liang B, Roller J, Li C, Li A, Tieleman P, Van Petegem F, Tibbits G (2016) Functional Characterization of Cardiac and Slow Skeletal Troponin C Paralogs in Zebrafish by MD Simulation and Isothermal Titration Calorimetry. Biophysical J. 111, 38-49.

 

Ng L, Putrenko I, Baronas V, Van Petegem F, Accili E (2016) Cyclic purine and pyrimidine nucleotides bind to the HCN2 ion channel and variably promote C-terminal domain interactions and opening. Structure. 24,1629-1642

 

Van Petegem F (2016) “How to open a Ryanodine Receptor”. Cell Research. 26,1073-1074.

 

Roston T, Guo W, Krahn AD, Wang R, Van Petegem F, Sanatani S, Chen SR, Lehman A (2016) A Novel RYR2 Loss-of-Function Mutation (I4855M) is Associated with Left Ventricular Non-compaction and Atypical Catecholaminergic Polymorphic Ventricular Tachycardia. J. Electrocardiol. In press.

 

McGregor N, Yin V, Ching-Chieh T, Van Petegem F, Brumer H (2016) Crystallographic insight into the evolutionary origins of xyloglucan endo-transglycosylases and endo-hydrolases. The Plant J.In Press.

 

Roston T, Van Petegem F, Sanatani S (2016) Catecholaminergic Polymorphic Ventricular Tachycardia: a model for Genotype-Specific Therapy. Curr.Opinion in Cardiol. In Press.

 

Van Petegem F (2016) “Ligand binding to Ryanodine Receptors revealed through cryo-electron microscopy”. Cell Calcium. In Press.

 

Sondergaard MT, Liu Y, Larsen KT, Nani A, Tian X, Holt C, Wang R, Wimmer R, Van Petegem F, Fill M, Chen SR, Overgaard MT (2016) The Arrhythmogenic Calmodulin p.Phe142Leu Mutation Impairs C-domain Ca2+-binding but not Calmodulin-dependent Inhibition of the Cardiac Ryanodine Receptor. J. Biol. Chem. In Press.

 

Roston T, Yuchi Z, Kannankeril PJ, Hathaway J, Vinocur JM, Etheridge SP, Potts JE, Maginot KR, Salerno JC, Cohen M, Hamilton RM, Pflaumer A, Mohammed S, Kimlicka L, Kanter RJ, LaPage MJ, Collins KK, Gebauer RA, Temple JD, Batra AS, Erickson C, Miszczak-Knecht M, Kubus P, Bar-Cohen Y, Kantoch M, Thomas VC, Hessling G, Anderson C, Young ML, Choi SHJ, Cabrera Ortega M, Lau YR, Johnsrude CL, Fournier A, Van Petegem F, Sanatani S (2016). The Clinical and Genetic Spectrum of  Catecholaminergic Polymorphic Ventricular Tachycardia: Identifying Predictors of Arrhythmia in an International Multicenter Registry. Europace. In Press.

 

 

2015

Xiao Z, Guo W, Wong King Yuen S, Wang R, Zhang L, Van Petegem F, Chen SR (2015) The H29D mutation does not enhance cytosolic Ca2+ activation of the cardiac Ryanodine Receptor. Plos One 10:e0139058.

 

Brath U, Swamy SI, Veiga AX, Tung CC, Van Petegem F, Erdelyi M (2015) Paramagnetic Ligand Tagging to Identify Protein Binding Sites. J. Am. Chem. Soc.137,11391-11398.

 

Yuchi Z, Wong King Yuen S, Lau K, Underhill AQ, Cornea R, Fessenden J, Van Petegem F (2015) Crystal structures of Ryanodine Receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant. Nature Comm. 6,7947.

 

Liu Y, Xiao Z, Wang R, Guo W, Zhang JZ, Mi T, Wang Y, Jones PP, Van Petegem F, Chen SRW (2015) Roles of the NH2-terminal Domains of Cardiac Ryanodine Receptor in Ca2+ Release Activation and Termination. J. Biol. Chem. 290, 7736-7743.

Van Petegem F (2015). Ryanodine Receptors: Allosteric Ion Channel Giants. J. Mol. Biol. 427,31-53

2014

Lau K, Van Petegem F (2014) Crystal structures of wild type and disease mutant forms of the Ryanodine Receptor SPRY2 domain.Nature Comm. 5:5397

 

Trane AE, Pavlov D, Sharma A, Saqib U, Lau K, Van Petegem F, Minshall RD, Roman LJ, Bernatchez PN (2014) Deciphering the binding of caveolin-1 to clien protein endothelial nitric-oxide synthase (eNOS): scaffolding subdomain identification, interaction modeling, and biological significance. J Biol Chem 289, 13273-13283.

 

Lau K, Chan MM, Van Petegem F* (2014) Lobe-Specific Calmodulin Binding to Different Ryanodine Receptor Isoforms. Biochemistry 53, 932-946

 

Brath U, Lau K, Van Petegem F*, Erdelyi M (2014) Mapping the sevoflurane-binding sites of calmodulin. Pharma Res Per, 2, e00025 doi:10.1002/prp2.25.

 

2013

beta4

Gilchrist J, Das S, Van Petegem F*, Bosmans F* (2013) Crystallographic insights into sodium-channel modulation by the β4 subunit. Proc. Natl. Acad. Sci. USA. 110, E5016-5024. *shared corresponding authorship

 

Zhong X, Liu Y, Zhu L, Meng X, Wang R, Van Petegem F, Wagenknecht T, Chen SR, Liu Z (2013) Conformational dynamics inside amino-terminal disease hotspot of ryanodine receptor. Structure 21, 2051-2060.

 

Ellis SJ, Goult BT, Fairchild MJ, Harris NJ, Long J, Lobo P, Czerniecki S, Van Petegem F, Schöck F, Peifer M, Tanentzapf G. (2013) Talin autoinhibition is required for morphogenesis. Curr Biol 23,1825-1833.

 

Amador FJ, Kimlicka L, Stathopulos PB, Gasmi-Seabrook GM, Maclennan DH, Van Petegem F*, Ikura M* (2013) Type 2 ryanodine receptor domain A contains a unique and dynamic α-helix that transitions to a β-strand in a mutant linked with a heritable cardiomyopathy. J Mol Biol 425,4034-4046 *Shared corresponding authorship.

 

Kimlicka L, Tung CC, Carlsson AC, Lobo PA, Yuchi Z, Van Petegem F (2013) The cardiac ryanodine receptor N-terminal region contains an anion binding site that is targeted by disease mutations. Structure 21,1440-1449.

 

Liu Y, Kimlicka L, Hiess F, Tian X, Wang R, Zhang L, Jones PP, Van Petegem F, Chen SR (2013) The CPVT-associated RyR2 mutation G230C enhances store overload-induced Ca2+ release and destabilizes the N-terminal domains. Biochem J 454,123-131

 

Kimlicka L, Lau K, Tung CC, Van Petegem F (2013) Disease Mutations in the Ryanodine Receptor N-terminal region couple to a mobile intersubunit interface. Nature Comm. 4:1506.

 

openclose

2012

Van Petegem F, Lobo PA, Ahern CA (2012) Seeing the forest through the trees: towards a unified view on physiological calcium regulation of voltage-gated sodium channels. Biophys J 103, 2243-2251.

 

JBCover

Van Petegem F (2012) Ryanodine Receptors: Structure and Function. J. Biol. Chem.287, 31624-31632

 

Yuchi Z, Lau K, Van Petegem F (2012) Disease Mutations in the Ryanodine Receptor Central Region: Crystal Structures of a Phosphorylation Hot Spot Domain. Structure. 20, 1201-1211.

 

Liu X, Chen M, Lobo P, An J, Grace Cheng SW, Moradian A, Morin GB, Van Petegem F*, Jiang X* (2012) Molecular and structural characterization of the SH3 domain of AHI-1 in regulation of cellular resistance of BCR-ABL(+) chronic myeloid leukemia cells to tyrosine kinase inhibitors. Proteomics 12 ,2094-2106 *co-corresponding authors

 

sodium

 

Sarhan MF, Tung CC, Van Petegem F*, Ahern CA* (2012) Crystallographic basis for calcium regulation of sodium channels. Proc Nat Acad Sci USA 109,3558-3363. *co-corresponding authors

 

Chow SS, Van Petegem F, Accili EA (2012) Energetics of cAMP binding to HCN terminus reveal negative cooperativity. J. Biol. chem. 287, 600-606.

 

2011

ScienceChina-cover

Kimlicka L, Van Petegem F(2011) The structural biology of ryanodine receptors. Science China Life Sciences. 54, 712-724. (review article)

 

Lobo PA, Kimlicka L, Tung CC, Van Petegem F (2011) The RyR2 exon3 deletion is rescued by beta strand switching. Structure 19,90-98.

 

Yuchi Z, Van Petegem F (2011) Common allosteric mechanisms between ryanodine and inositol-1,4,5-trisphosphate receptors. Channels 5, 120-123.

 

2010

Nter

Tung CC, Lobo PA, Kimlicka L, Van Petegem F (2010) The N-terminal disease hot spot of Ryanodine Receptors forms a cytoplasmic vestibule. Nature. 468, 585-588.

 

Kim EY, Rumpf CH, Van Petegem F, Arant RJ, Findeisen F, Cooley ES, Isacoff EY, Minor DL Jr. (2010) Multiple C-terminal tail Ca(2+)/CaMs regulate Ca(V)1.2 function but do not mediate channel dimerization. EMBO J. 29,3924-3938.

 

Okamoto S, Van Petegem F, Patrauchan MA, Eltis LD (2010) AnhE, a metallochaperone involved in the maturation of a cobalt-dependent nitrile hydratase. J Biol Chem. 285, 25126-25133.

 

Janssens ME, Geysen D, Broos K, De Goeyse I, Robbens J, Van Petegem F, Timmermans J-P, Guisez Y (2010) Folding properties of the hepatitis B core as a carrier protein for vaccination research. Amino Acids. 38, 1617-1626.

 

2009

Cheung TYS, Fairchild M, Zarivach R, Tanentzapf G, Van Petegem F (2009) Crystal Structure of the Talin Integrin Binding Domain 2. J. Mol. Biol. 387, 787-793.

 

Sarhan MS, Van Petegem F, Ahern CA (2009). A double tyrosine motif in the cardiac sodium channel Domain III-IV linker couples calcium dependent calmodulin binding to inactivation gating. J Biol Chem, 284, 33265-33274

 

Lobo PA, Van Petegem F (2009). Crystal structures of the N-terminal domains of cardiac and skeletal muscle ryanodine receptors: insights into disease mutations. Structure, 17,1505-1514.

 

2008

De Vos D, Xu Y, Aerts T, Van Petegem F, Van Beeumen JJ (2008). Crystal structure of Sulfolobus acidocaldarius aspartate carbamoyltransferase in complex with its allosteric activator CTP. Biochem Biophys Res Commun., 372, 40-44

 

Kim EY, Rumpf CH, Fujiwara Y, Cooley ES, Van Petegem F, Minor DL (2008). Structures of CaV2 Ca2+/CaM-IQ domain complexes reveal binding modes that underlie calcium-dependent inactivation and facilitation. Structure, 16, 1455-1467.

 

Van Petegem F, Duderstadt KE, Clark KA, Wang M, Minor DL (2008). Alanine-scanning mutagenesis defines a conserved energetic hotspot in the CaVα AID-CaVβ interaction site that is critical for channel modulation. Structure, 16, 280-294.

 

2005-2007

Van Petegem F, De Vos D, Savvides S, Vergauwen B, Van Beeumen J (2007). Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: Crystallographic analysis of a glutathione amide J. Mol. Biol., 374, 883-889

 

Van Petegem F, Minor DL (2006). The structural biology of voltage-gated calcium channel function and regulation. Biochem. Soc. Trans, 34, 887-893.

 

Van Petegem F, Chatelain FC, Minor DL (2005) Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex. Nature Struct. Mol. Biol., 12, 1108-1115

 

2003-2004

Van Petegem F, Clark KA, Chatelain FC, Minor DL Jr. (2004) Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain. Nature 429, 671-675.

 

Dumortier C, Fitch J, Van Petegem F, Vermeulen W, Meyer TE, Van Beeumen J, Cusanovich MA. (2004) Protein dynamics in the region of the sixth ligand methionine revealed by studies of imidazole binding to Rhodobacter capsulatus cytochrome c2 hinge mutants. Biochemistry, 43, 7717-7724.

 

De Vos D, Van Petegem F, Remaut H, Legrain C, Glansdorff N, Van Beeumen J. (2004) Crystal structure of T state aspartate carbamoyltransferase of the hyperthermophilic archaeon Sulfolobus acidocaldarius. J. Mol. Biol. 339, 887-900.

 

Aghajari N, Van Petegem F, Villeret V, Chessa JP, Gerday C, Haser R, and Van Beeumen J. (2003). Crystal structures of a psychrophilic metalloprotease provide new insights into catalysis by cold-adapted proteases. Proteins: Struct. Funct. Genet. 50, 636-647.

 

2000-2002

Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, and Van Beeumen J (2002). The structure of a cold-adapted family 8 xylanase at 1.3 Å resolution, structural adaptations to cold and investigation of the active site. J. Biol. Chem., 278, 7531-7539

 

Van Petegem F, Vandenberghe I, Bhat MK, and Van Beeumen J (2002). Atomic resolution structure of the major endoglucanase from normal Thermoascus aurantiacus. Biochem. Biophys. Res. Commun. 296, 161-166.

 

Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, and Van Beeumen J (2002). Crystallization and preliminary X-ray analysis of a xylanase from the psychrophile Pseudoalteromonas haloplanktis. Acta Cryst. D., 58, 1494-1496.

 

Vergauwen B, Van Petegem F, Remaut H, Pauwels F, and Van Beeumen J (2002). Crystallization and preliminary X-ray crystallographic analysis of glutathione amide reductase from Chromatium gracile. Acta Cryst. D., 58, 339-340.

 

Van Petegem F, Contreras H, Contreras R, and Van Beeumen J (2001). Trichoderma reesei alpha-1,2-mannosidase: structural basis for the cleavage of four consecutive mannose residues. J. Mol. Biol., 312, 157-165.

 

Georlette D, Jonsson ZO, Van Petegem F, Chessa J, Van Beeumen J, Hubscher U, Gerday C (2000). A DNA ligase from the psychrophile Pseudoalteromonas haloplanktis gives insights into the adaptation of proteins to low temperatures. Eur. J. Biochem. 267, 3502-3512.

keywords: cardiac arrhythmia, epilepsy, ion channel, CPVT, Dravet syndrome, malignant hyperthermia, central core disease, excitation-contraction coupling, ryanodine receptor, sodium channel, calcium channel, voltage-gated sodium channel, calmodulin, X-ray crystallography, ryanodine, RyR, RyR1, RyR2, RyR3, high resolution, structure, arrhythmia, genetic disorder, ITC, isothermal titration calorimetry, Brugada syndrome, Long QT syndrome, arrhythmia, channel, electrophysiology, GEFS+, Vancouver, UBC, Biochemistry, Canada
tel:604.827.4267 | fax:604.822.5227 | email:filip.vanpetegem'at'gmail.com

Van Petegem Lab © 2012, updated: Feb/2016